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TARGET-seq+ metadata

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posted on 2024-06-24, 11:44 authored by Niels Asger JakobsenNiels Asger Jakobsen, Sven Turkalj, Paresh Vyas
<p dir="ltr">Metadata for TARGET-seq+ single-cell whole transcriptome data from bone marrow samples from 9 cases with clonal hematopoiesis and 4 control samples. For details on cell sorting and the TARGET-seq+ protocol see the methods section of the manuscript. Cells are named with their plate and well IDs and the subject ID. Empty wells (no-cell controls) are named "blank".</p><h3>Column descriptions</h3><p dir="ltr">Column 1 ('Cell'): Cell ID</p><p dir="ltr">Column 2 ('Sample'): Donor ID</p><p dir="ltr">Column 3 ('Sex'): Donor sex</p><p dir="ltr">Column 4 ('Age'): Donor age</p><p dir="ltr">Column 5 ('Sample_type'): Type of sample; CH = clonal haematopoiesis sample; WT_control = non-CH wild-type control sample; No_template_control = empty well control</p><p dir="ltr">Column 6 ('Sort'): FACS sort ID</p><p dir="ltr">Column 7 ('Plate'): = Plate ID</p><p dir="ltr">Columns 8-24: FACS indexing expression profiles.</p><p dir="ltr">Column 25 ('Single_cell'): indicates whether single cell sorted into well; FALSE indicates wells with more than one cell (excluded from analysis)</p><p dir="ltr">Columns 26-36: FACS profiles and immunophenotypic population based on gating on FACS indexing expression values</p><p dir="ltr">Column 37 ('Genotype'): Clonal identity assigned based on single-cell genotyping</p><p dir="ltr">Column 38 ('Cluster'): Cell type based on transcriptome clustering</p><p dir="ltr">Column 39 ('PassedQC'): Whether the cell passed transcriptome quality control</p>

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