TARGET-seq+ genotyping data

<p dir="ltr">Genotyping data for TARGET-seq+ from bone marrow samples from 9 cases with clonal hematopoiesis and 1 control sample.</p><p dir="ltr">For each mutation and each single cell, data are provided for the genomic DNA amplicon (".gDNA"), the mRNA/cDNA amplicon (".mRNA"; if present) and the heterozygous germline SNP ("SNP."; if present). Where multiple mutations were amplified in a single cell, data for these are shown in separate lines.</p><p dir="ltr">Genotypes were called separately for the genomic DNA amplicon ("genotype.gDNA"), and the mRNA/cDNA amplicon ("genotype.mRNA"). Zygosity was called for heterozygous SNPs where these were present in the amplicon.</p><p dir="ltr">The final column indicates the consensus cell genotype for that mutation, integrating information from gDNA, mRNA and heterozygous SNPs.</p><h3><u>Column descriptions</u></h3><p dir="ltr">Cell = Cell ID</p><p dir="ltr">Sample = Donor ID</p><p dir="ltr">Sample_type = Type of sample; CH = clonal haematopoiesis sample; WT_control = non-CH wild-type control sample; No_template_control = empty well control</p><p dir="ltr">Plate = Plate ID</p><p dir="ltr">mutation = mutation amplified</p><p dir="ltr">coverage.gDNA = total coverage of locus in gDNA amplicon</p><p dir="ltr">Ref.gDNA = reference allele count in gDNA amplicon</p><p dir="ltr">Mut.gDNA = mutant allele count in gDNA amplicon</p><p dir="ltr">VAF.gDNA = variant allele frequency in gDNA amplicon</p><p dir="ltr">genotype.gDNA = genotype called in gDNA amplicon</p><p dir="ltr">coverage.mRNA = total coverage of locus in mRNA amplicon</p><p dir="ltr">Ref.mRNA = reference allele count in mRNA amplicon</p><p dir="ltr">Mut.mRNA = mutant allele count in mRNA amplicon</p><p dir="ltr">VAF.mRNA = variant allele frequency in mRNA amplicon</p><p dir="ltr">genotype.mRNA = genotype called in mRNA amplicon</p><p dir="ltr">SNP = Name of the germline SNP (if present) found near the mutation locus, covered by the gDNA amplicon</p><p dir="ltr">SNP.coverage = total coverage across the SNP in the gDNA amplicon</p><p dir="ltr">SNP.ref = reference allele count for the SNP</p><p dir="ltr">SNP.alt = alternate allele count for the SNP</p><p dir="ltr">SNP.VAF = variant allele frequency for the SNP (alternate allele)</p><p dir="ltr">SNP.genotype = genotype called for the SNP; Het = Heterozygous biallelic detection; Hom_Ref = homozygous reference; Hom_Alt = homozygous alternate</p><p dir="ltr">genotype = consensus genotype</p>